Journal: Scientific Reports

Article Title: Oxidative stress promotes the progression of age-related macular degeneration by up-regulating Spp1 to activate the JAK/STAT signaling pathway

doi: 10.1038/s41598-026-48556-6

Figure Lengend Snippet: Verification of oxidative stress in RPE cells. ( A ) Immunofluorescence double staining showing the co-localization of SPP1 and the RPE cell marker RPE65; ( B ) Immunofluorescence double staining showing the co-localization of SPP1 and the microglial marker IBA1; ( C-D ) Immunofluorescence detection of RPE cell markers (RPE65, BEST1) under oxidative stress and SPP1 knockdown conditions; ( E ) ROS levels in the Control and H 2 O 2 groups; ( F ) GSH, SOD, and GPx levels in the Control and H 2 O 2 groups, ( G ) JAK2, STAT3, p-JAK2, and p-STAT3 levels in the Control, H 2 O 2 groups detected by WB; (H) Protein quantification of JAK2, STAT3, p-JAK2 and p-STAT3 in each group; ( I ) The survival rate of ARPE-19 cells in the Control, H 2 O 2 , H 2 O 2 +WP1066, and H 2 O 2 +IFN-γ groups detected by CCK-8.

Article Snippet: In the Control group, ARPE-19 cells were cultured with complete medium; In the H 2 O 2 group, ARPE-19 cells were induced with H 2 O 2 (200 μM) for 24 h. In the inhibitor group, H 2 O 2 -induced ARPE-19 cells were treated with WP1066 (MCE, HY-15312).

Techniques: Immunofluorescence, Double Staining, Marker, Knockdown, Control, CCK-8 Assay

Journal: Scientific Reports

Article Title: Oxidative stress promotes the progression of age-related macular degeneration by up-regulating Spp1 to activate the JAK/STAT signaling pathway

doi: 10.1038/s41598-026-48556-6

Figure Lengend Snippet: Verification of the effect of SPP1 expression on apoptosis of RPE cells. ( A ) The expression of SPP1 in ARPE-19 cells in the Control and H 2 O 2 groups detected by WB; ( B ) The expression of SPP1 in ARPE-19 cells in the H 2 O 2 and H 2 O 2 +si-SPP1 groups detected by qRT-PCR; ( C ) The knockdown efficiency of SPP1 was verified by qRT-PCR; ( D )ROS levels in the H 2 O 2 and H 2 O 2 +si-SPP1 groups; ( E ) Gsh, SOD and GPx levels in the H 2 O 2 and H 2 O 2 +si-SPP1 groups; ( F ) The expression of CD44 in the H 2 O 2 group and the H 2 O 2 +si-SPP1 group detected by WB; ( G ) The levels of JAK2, STAT3, p-JAK2 and p-STAT3 in the H 2 O 2 and H 2 O 2 +si-SPP1 groups detected by WB. ( H ) Protein quantification of JAK2, STAT3, p-JAK2 and p-STAT3 in each group; ( I ) The survival rate of ARPE-19 cells in the H 2 O 2 , H 2 O 2 +si-SPP1, H 2 O 2 +si-SPP1 + reco-SPP1, H 2 O 2 +WP1066, and H 2 O 2 +IFN-γ groups detected by CCK-8. Compared with the control group, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; # # P < 0.01.

Article Snippet: In the Control group, ARPE-19 cells were cultured with complete medium; In the H 2 O 2 group, ARPE-19 cells were induced with H 2 O 2 (200 μM) for 24 h. In the inhibitor group, H 2 O 2 -induced ARPE-19 cells were treated with WP1066 (MCE, HY-15312).

Techniques: Expressing, Control, Quantitative RT-PCR, Knockdown, CCK-8 Assay

Journal: Cancer Medicine

Article Title: FCGBP Promotes Glioma Growth by Regulating JAK2 / STAT3 /c‐Myc Pathway

doi: 10.1002/cam4.71617

Figure Lengend Snippet: FCGBP promotes glioma growth by activating the JAK2/STAT3 signaling pathway. (A) WB analysis was performed to evaluate the expression levels of JAK2, p‐JAK2, STAT3, p‐STAT3, and c‐Myc in Vector, oeFCGBP, Vector + WP1066, and oeFCGBP + WP1066 groups. (B) Cell growth curves of different treatment groups were measured using the CCK‐8 assay. (C) EdU incorporation assay and corresponding histogram quantification were used to evaluate cell proliferation across different treatment conditions. Data were mean ± SD. Statistical significance was calculated by 2‐way ANOVA for A–C. ** p < 0.01, *** p < 0.001.

Article Snippet: The compound WP1066 (S2796) was sourced from Selleck, China.

Techniques: Expressing, Plasmid Preparation, CCK-8 Assay